FIGURE 7.

porin mutants have reduced mitochondria in NMJ presynaptic termini. A, labeling of third instar larval NMJs with Dlg (blue) to outline boutons, GluR3 (GluRIII/IIC, red) to demarcate postsynaptic receptor field, and mitoGFP (green) expressed in motor neurons. B, quantification of fluorescence per field demonstrates decreased mitochondria in porin^Rev8. Error bars represent S.E. *, p < 0.05 by Student's t test with Welch correction. C, third instar larval ventral nerve cords labeled with Dlg (blue), which labels the neuropil (np) and motor axons (ma), and with mitoGFP (green). In porin^Rev8 animals, mitoGFP is more prominent in the cell bodies (cb) and sparser in the neuropil and motor axons compared with wild type (yw). Rev8, porin^Rev8.