FIGURE 6.

CaMKII does not activate a chloride current after ClC-3 knockdown. A, ClC-3 expression is decreased in H8a cells expressing ClC-3 small hairpin RNA relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) loading control, and CaMKII expression is not altered. B–E, using whole-cell patch clamp electrophysiology, human glioma cells were held at −40 mV and stepped from −100 mV to +120 mV. For the representative traces, the 1st column (a) is basal current at 1 min, and the 2nd column (b) is current at 21 min. At 21 min, NPPB was added as seen in the 3rd column (c), and the NPPB-sensitive current (b − c) is depicted in the 4th column. B, control condition. C, activated CaMKII included in the pipette solution does not lead to an enhancement of chloride current. D, peak whole-cell current density corresponding to b. E, peak NPPB-sensitive current density corresponding to b and c. n = 5–6.