FIGURE 8.

ClC-3 is overexpressed in grade IV human glioma tissue and associated with CaMKII. A, representative blot demonstrating ClC-3 and CaMKII immunoreactivity in normal brain, grade II, grade III, and grade IV human biopsy samples. B, quantification of ClC-3 expression normalized to normal brain reveals overexpression in grade IV samples. (*, p < 0.05; Tukey Kramer). (Normal brain, n = 3; grade II, n = 4; grade III, n = 3; and grade IV, n = 4.) C, after immunoprecipitation of ClC-3 from a grade IV glioblastoma patient biopsy, CaMKII was detected only when the precipitating antibody was included. D, after immunoprecipitation of CaMKII from a grade IV glioblastoma patient biopsy, ClC-3 was detected only when the precipitating antibody was included. Blots are representative of n ≥ 3 experiments.