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. 2010 Feb 4;285(15):11219–11226. doi: 10.1074/jbc.M109.042754

FIGURE 2.

FIGURE 2.

HDAC6 knock-down reduces EGFR stability. A, A549 cells with stable HDAC6 knock-down were examined by immunoblotting for EGFR, HDAC6, acetyl-tubulin, and β-actin. B, A549 cells were transfected with control siRNA or HDAC6-targeting siRNA for 2, 3, or 4 days. Cell lysates from both control and HDAC6 siRNA-treated cells were prepared and analyzed by immunoblotting using anti-HDAC6, anti-EGFR, anti-Erk1/2, and anti-acetyl α-tubulin antibodies. EGFR level was reduced in HDAC6 knock-down cells. C, LNCaP and Panc-1 cells were transfected with control siRNA or HDAC6-targeting siRNA for 3 days. Cell lysates from both control and HDAC6 knock-down cells were analyzed by immunoblotting using anti-HDAC6, anti-EGFR, anti-β-actin, and anti-acetyl α-tubulin antibodies. *, unidentified band. D, HDAC6 was transiently knocked down for 3 or 4 days as in B. EGFR transcripts were prepared and amplified by real-time PCR. EGFR mRNA from HDAC6 knock-down cells was compared with that from control cells. Average values from triplicate wells are shown.