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Hydrophobicity of Hsp33 and variants. 3 μm freshly reduced wild-type Hsp33red (trace a), Hsp33-M172S red (trace b), Hsp33-Y12Ered (trace c), or active wild-type Hsp33ox43 °C (trace a′), Hsp33-M172S ox43 °C (trace b′), Hsp33-Y12E ox43 °C (trace c′) were incubated with 10 μm of the hydrophobic probe bis-ANS in 40 mm Hepes buffer, pH 7.5. Fluorescence spectra were recorded and buffer corrected.
