FIGURE 1.

WDR20 interacts with UAF1, USP12, and USP46, but not with USP1. A, schematic of the domain distribution of WDR20 and UAF1 is shown. Dark boxes indicate the WD40-repeat motifs. B, anti-FLAG immunoprecipitations (IP) were performed using the lysates from HeLa cells stably expressing each FLAG-HA tandem-tagged proteins, followed by Western blots using anti-FLAG, anti-UAF1, and anti-WDR20 antibodies. C, anti-FLAG immunoprecipitations were performed from HeLa cells stably expressing FLAG-HA-tagged WDR20 followed by Western blotting using anti-UAF1, anti-USP1, anti-USP12, and anti-FLAG antibodies. Note that there are two forms of USP1, with the full-length (long form) and C-terminally cleaved product (short form) of USP1, which is also detected by our anti-USP1 antibody, as described previously (21).