FIGURE 1.
[3H]5-HT transport activity of hSERT TM6 Cys mutants. hSERT Cys mutants cDNAs were transiently transfected in HEK-293T cells at equal concentrations and assayed for uptake at a single 3H-5-HT concentration (see “Experimental Procedures”). Results represent the means ± S.E. from three experiments. Means of Cys mutant uptake were compared with C109A using a one-way ANOVA with a post hoc Dunnett's test, with p < 0.05 indicating significance. #, inactive mutant. *, p < 0.05 compared with C109A. The dashed line indicates 5% C109A uptake activity. Homology model of hSERT TM6 is depicted as constructed by Kaufmann et al. (32) based on LeuTAa structures. Residues that are intolerant of Cys substitution are depicted in black.