FIGURE 2.

Total protein and surface expression of hSERT TM6 Cys mutants. To assess the impact of Cys substitution on TM6, whole cell and NHS-SS-biotinylated extracts were examined by Western blot using the monoclonal hSERT antibody ST51–2. Dashed lines indicate separate acrylamide gels run concurrently. *, mutant exhibiting less than 5% 5-HT uptake activity compared with C109A and no detectable surface expression. ↑, mutant exhibiting less than 5% 5-HT uptake activity compared with C109A but significant surface expression.