TABLE 1.
hSERT mutant | Km | Vmax |
---|---|---|
μm | fmol/cell/min | |
C109A | 1.93 ± 0.33 | 8.89 ± 0.67 |
T323C | 2.35 ± 0.82 | 14.3 ± 2.36a |
G324C | 2.10 ± 0.44 | 6.29 ± 0.60b |
V325C | 2.96 ± 0.73 | 5.90 ± 0.75b |
I327C | 1.25 ± 0.53 | 7.99 ± 1.32 |
D328C | 2.38 ± 1.00 | 2.26 ± 0.45b |
A330C | 1.61 ± 0.31 | 11.4 ± 0.92a |
A331C | 2.04 ± 0.43 | 19.2 ± 1.83a |
I333C | 9.49 ± 3.01a | 20.9 ± 4.88a |
F334C | 5.38 ± 2.40 | 41.8 ± 11.5a |
F335C | 2.91 ± 0.72 | 11.5 ± 1.45 |
S336C | 3.49 ± 1.47 | 0.32 ± 0.07b |
L337C | 8.62 ± 5.11 | 19.9 ± 8.22 |
P339C | 0.84 ± 0.18b | 4.36 ± 0.33b |
G340C | 0.84 ± 0.10b | 6.27 ± 0.27b |
V343C | 1.40 ± 0.57 | 6.31 ± 1.03 |
L344C | 0.55 ± 0.24b | 3.20 ± 0.44b |
L345C | 0.99 ± 0.42 | 5.93 ± 0.90b |
A346C | 1.34 ± 0.54 | 11.6 ± 1.84 |
F347C | 0.16 ± 0.13b | 1.66 ± 0.29b |
A348C | 0.19 ± 0.08b | 1.84 ± 0.17b |
a p < 0.05 above C109A.
b p < 0.05 below C109A.