FIGURE 1.

SL gene encodes a cytochrome P450 monooxygenase. A, structure of the SL gene. Arrows indicate the locations of mutations found in the sl mutants. Arrowheads indicate locations of primers used in B. aa, amino acids. B, expression of SL in the sl mutants. Reverse transcription-PCR was performed using specific primers for SL and ubiquitin. WT, wild type. C, subcellular localization of SL-GFP. GFP (upper panel) and SL-GFP (lower panel) were transiently expressed in rice protoplasts with ER-localized SECFP. Left panel, GFP image; middle panel, SECFP image; right panel, merged images of GFP and SECFP. Bars, 10 μm. D, expression of SL after elicitor treatment. Total RNAs were purified from rice suspension cultured cells either mock-treated or treated with 2 μg/ml N-acetylchitooligosaccharide elicitor and used for real time PCR. Ubiquitin (Ubq) was used as an internal control. Error bars indicate standard deviations. E, expression of SL in seedlings after mock inoculation or inoculation with compatible rice blast fungus (race 003 and 007). Real time PCR was carried out using total RNAs prepared from the seedlings. Ubq was used as an internal control. Error bars indicate S.D.