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. 2010 Feb 11;285(15):11308–11313. doi: 10.1074/jbc.M109.091371

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — SL is a tryptamine 5-hydroxylase. A, production of recombinant SL proteins in insect cells using a baculovirus expression system. Microsomal fractions from the insect cells expressing the recombinant SL proteins were separated by SDS-PAGE and analyzed on an immunoblot using the anti-SL antibody. CBB, Coomassie Brilliant Blue. B, CO difference spectrum of the microsomal fractions expressing SL proteins. C, HPLC analysis of reaction products after the microsomal SL proteins were assayed for enzyme activity using tryptamine as a substrate.