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. 2010 Feb 10;285(15):11314–11325. doi: 10.1074/jbc.M109.044057

FIGURE 4.

FIGURE 4.

Endogenous UCH-L1 levels are positively correlated with CFTR biogenesis. IB3-1 cells were transfected with WT or ΔF508 CFTR and a nontargeting siRNA (siCTRL) or siRNA targeting UCH-L1 for 48 h. Following transfection, CFTR biogenesis was analyzed by pulse-chase. UCH-L1 levels were examined by immunoblot, and actin served as a loading control. A, knockdown of UCH-L1 reduces WT CFTR protein expression. A pulse-chase examining WT CFTR expression following UCH-L1 knockdown was performed as described under “Experimental Procedures.” B, densitometric analysis of immature band B WT CFTR from A. C, densitometric analysis of mature band C CFTR in A. D, knockdown of UCH-L1 with siRNA reduces ΔF508 CFTR protein expression. ΔF508 CFTR biogenesis was analyzed by pulse-chase. E, the mean ΔF508 CFTR expression ± S.E. from four independent experiments was calculated by densitometry. *, p < 0.05.