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. 2010 Feb 10;285(15):11314–11325. doi: 10.1074/jbc.M109.044057

FIGURE 8.

FIGURE 8.

The shortening of polyubiquitin chains via lysine-less ubiquitin (K0-Ub) expression stabilizes an early CFTR intermediate, similar to UCH-L1. S9 cells were co-transfected with ΔF508 CFTR, UCH-L1, and K0-Ub or their vector controls (pEGFP-C1, pcDNA3.1, and pRK5, respectively) as indicated for 24 h. A pulse-chase examining ΔF508 CFTR biogenesis was performed as described under “Experimental Procedures.” The cells were lysed in RIPA and immunoprecipitated for CFTR. A, UCH-L1 and K0-Ub overexpression similarly increase the amount of ΔF508 CFTR synthesized during a 20-min pulse. Radiolabeled ΔF508 CFTR was analyzed by autoradiography. B, densitometric analysis of ΔF508 CFTR levels displayed in A.

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