TABLE 2.

Analysis of the expression level of genes of interest

The expression level of genes of interest was determined in WT and Δpim1 cells by quantitative PCR experiments, as described under “Experimental Procedures.” For each gene, relative expression (Δpim1/WT) was determined by calculating the ratio between the expression level from Δpim1 and WT. Sequences of primers used are depicted: the first is the forward primer, and the second is the reverse primer.

Genes	Primers (5′–3′)	Expression level in WT	Expression level in Δpim1	Relative expression Δpim1/WT
HSP78	CGACGGTAAATTAGACCCTGTC	0.84 ± 0.07	1.54 ± 0.22	1.83
	ATTTGAATAGCTCTTGCGATTTCT
HSP60	GAAGTTGCCTCCAAAACCAA	0.89 ± 0.05	2.15 ± 0.18	2.41
	TGGCTCTACCTAAGACAGTAGCAG
SOD2	AAACCACTGTCTATTCTGGGAAA	0.90 ± 0.19	1.34 ± 0.06	1.49
	CTCGTCGATTGCCTTTGC
PDB1	ATACGGGTTCACAGGTTTGG	0.88 ± 0.05	0.74 ± 0.04	0.84
	GCTTGCATAGAGAAATTGAACG
LAT1	AAAGACCAGTACGAAGGCTCAG	0.88 ± 0.05	0.76 ± 0.08	0.86
	GCTGGCGCTTCTTGTTTCT
LPD1	TTCGTTAAAGGAAATTCCCAAA	0.86 ± 0.53	0.68 ± 0.05	0.79
	ACCCATTTCCAATCCGATG
COQ5	TTACGCAAGCTCACAGAGCA	1.39 ± 0.244	1.17 ± 0.17	0.84
	GCTGCGGATGATAAAGGACT
DLD1	TATTTGAGCGACCACGGTTT	0.73 ± 0.04	0.48 ± 0.05	0.66
	AACCACCAATCTGTGCACCT
ALD4	CGGGTACGGTCTGGATAAAC	1.36 ± 0.29	1.74 ± 0.22	1.28
	AACCCACCGAAAGGAACTG
ILV5	GCCATTGTTGACCAAGGGTA	0.73 ± 0.16	0.64 ± 0.10	0.88
	TCCTTGAAGACTGGGGAGAA
QCR2	GCGGCCTATTTACTCTGTTTGT	0.97 ± 0.17	1.17 ± 0.13	1.21
	CCGCAACAATTTTCTTGATGT
RIP1	TGTGTTCCAATTGGTGAAGC	0.85 ± 0.31	1.35 ± 0.30	1.59
	TGAACCATGGCAAGGACAG
ATP1	CTCCTGGTCGTGAAGCCTAC	1.17 ± 0.25	1.17 ± 0.04	1.00
	GCGGCTCTTTCTAGCAATCTT
ATP2	TCCGTGGTGAAAAGGTTCTT	0.93 ± 0.05	0.90 ± 0.02	0.97
	TCCCTAAAGTTTCTCTCCCAACT
MRP20	GCCAGAGCAGGTTCCAATAA	1.25 ± 0.27	0.56 ± 0.43	0.45
	GCCGCTTTTGTCGAGTATTT