FIGURE 4.

Meiotic DSB resection involves both Exo1 and Sgs1. A–C, synchronous meiotic cultures of cells with the indicated genotypes were analyzed at the indicated times for DNA content by FACS (A) and for DSB resection by Southern blot (B) as described in Fig. 3C. C, densitometric analysis of the representative experiment shown in B. Values are expressed as arbitrary units. Three independent experiments were performed with very similar results. D, Spo11-DNA association. Synchronous meiotic cultures of cells with the indicated genotypes were analyzed at the indicated times by ChIP and quantitative real-time PCR as described in Fig. 3A.