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. 2010 Feb 8;285(15):11638–11651. doi: 10.1074/jbc.M109.045963

FIGURE 3.

FIGURE 3.

TβRI and JNK activities are required to regulate TGF-β1-induced FXII expression in HLF. A, Western blot analysis of TGF-β1-induced FXII expression in HLF. HLF were treated with TβRI (SB431542; 8 μm), JNK (SP600125; 5 μm), PI3K (wortmannin; 80 nm), MEK (PD98059; 30 μm), or p38 (SB203580; 8 μm) inhibitors for 1 h prior to incubation with TGF-β1 for 48 h. Cell lysates were prepared, and FXII expression was examined. β-Actin was used as a loading control. The Western blot illustrated is from one representative experiment out of four. B, densitometric analysis of A. Data are presented as means ± S.D.; n = 4; **, p < 0.01. C and E, Western blot analysis of TGF-β1-induced FXII expression in HLF pretreated for 1 h with various concentrations of SB431542 (C) or SP600125 (E). β-Actin was used as a loading control. The Western blot illustrated is from one representative experiment out of three. D and F, densitometric analysis of C and E, respectively. Data are presented as mean ± S.D.; n = 3; *, p < 0.05; **, p < 0.01; ***,p < 0.001. wort, wortmannin; NS, not significant.