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. 2010 Feb 8;285(15):11638–11651. doi: 10.1074/jbc.M109.045963

FIGURE 4.

FIGURE 4.

Smad3 and JNK regulate TGF-β1-induced FXII expression in HLF. A and D, determination of knockdown efficiency in HLF after transfection with siRNA against (A) JNK or (D) Smad3 as assessed by Western blotting. Data are representative of three independent experiments. B and E, effect of JNK (B) or Smad3 (E) knockdown on TGF-β1-induced FXII expression in HLF. Data are representative of four independent experiments. C and F, densitometric analysis of B and E, respectively. Data are presented as means ± S.D.; n = 4; **, p < 0.01; ***, p < 0.001. G, impact of Smad3 knockdown on TGF-β1-induced JNK expression and phosphorylation. Data are representative of three independent experiments. H and I, densitometric analysis of G. Data are presented as means ± S.D.; n = 3. J, effect of Smad3 depletion on JNK translocation into the nucleus. Nuclear extracts were prepared and immunoblotted with antibodies against phospho-JNK, lamin B, and tubulin. Lamin B was used as a loading control, and tubulin was used to assess the purity of the nuclear fraction. Data are representative of three independent experiments. siR, scramble siRNA; NS, not significant.