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. 2010 Feb 8;285(15):11638–11651. doi: 10.1074/jbc.M109.045963

FIGURE 7.

FIGURE 7.

FXII promoter contains a repressor element located at the position −577/−541. A, schematic representation of FXII promoter luciferase reporter constructs. The angled arrow indicates the transcription start site. B, NIH3T3 cells were transfected with the indicated FXII promoter deletion constructs and then were either not stimulated (open columns) or were stimulated with TGF-β1 (closed columns). Luciferase activity is expressed in relative luminescence units (RLU). Data represent mean values ± S.D. from four independent experiments, each performed in triplicate; *, p < 0.05; **, p < 0.01. C, schematic representation of the pGL3-907 construct and the construct lacking repressor element (RE) located at the position −577/−541 (pGL3-907ΔRE). D, NIH3T3 cells were transfected with pGL3-907 or pGL3-907ΔRE, and the luciferase activity was measured in unstimulated (open columns) and TGF-β1-stimulated cells (closed columns). Data represent mean values ± S.D. from four independent experiments, each performed in triplicate; *, p < 0.05.