FIGURE 9.

Expression of FXII is elevated in the lungs of ARDS patients. A, quantification of FXII in BAL fluids from ARDS patients and healthy controls as assessed by immunoassay. The box-and-whiskers plots indicate the median, 1st, and 3rd quartile; the whiskers are extended to the most extreme value inside the 1.5-fold interquartile range. Significance levels are indicated. B, representative lung tissue sections from ARDS patient stained for FXII and vimentin. Arrows indicate fibroblast staining. Original magnification was ×20. Bar size, 200 μm. C, procoagulant activity of BAL fluids from ARDS patients was assessed by clotting assay in the absence or presence of FXII inhibitor (CTI), anti-TF blocking antibody (αTF), or an IgG isotype control (IgG). The box-and-whiskers plots indicate the median, 1st, and 3rd quartile; the whiskers are extended to the most extreme value inside the 1.5-fold interquartile range. Significance levels are indicated.