FIGURE 4.

PINK1^L347P induces mitochondrial translocation of Drp1. N27 cells were co-transfected with DsRed-Mito and Drp1-myc for 6 h before the addition of ponA for an additional 24 h. Cells were then fixed and immunostained for Myc. In control cells with empty vector (EV, A–C), with PINK1^WT overexpression (J–L), or with PINK1-knockdown after 48 h Drp1 appeared primarily diffused. However, in cells with PINK1^L347P overexpression (D–F), the distribution of Drp1 was punctated and co-localized with mitochondria. WT, wild type.