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. 2010 Feb 25;285(17):12916–12924. doi: 10.1074/jbc.M109.073924

FIGURE 1.

FIGURE 1.

Ror2 is regulated by VHL status. A, Ror2 is expressed when VHL is mutated in the 786-0 paired cell line. Whole cell extracts from 786-0 cells expressing (786-0 WT8) or lacking expression (786-0 RC3) of pVHL were immunoblotted with monoclonal Ror2 antibody (Ror2(1)), polyclonal Ror2 antibody (Ror2(2)), and Ku80 antibody as a loading control (LC). B, Ror2 expression is regulated at the transcriptional level in the paired 786-0 cell lines. qRT-PCR analysis data are normalized to β-actin as an RNA internal standard and displayed as expression levels relative to the pVHL expressing member of the cell line pair. Significant differences were observed as ROR2 mRNA levels were suppressed in the VHL(+) cell line (light gray), relative to the paired vector-transfected VHL(−) control (dark gray), **, p < 0.0001. C, Ror2 is expressed when VHL is mutated in the RCC4 paired cell line. Whole cell extracts from RCC4 cells expressing (RCC4 3-14) or lacking expression (RCC4 2-1) of pVHL were immunoblotted with monoclonal Ror2 antibody (Ror2(1)), polyclonal Ror2 antibody (Ror2(2)), and Ku80 antibody as a LC. D, Ror2 expression is regulated at the transcriptional level in the paired RCC4 cell lines. qRT-PCR analysis data are normalized to β-actin as an RNA internal standard and displayed as expression levels relative to the pVHL expressing member of the cell line pair. Significant differences observed as ROR2 mRNA levels were suppressed in the VHL(+) cell line (light gray), relative to the paired vector-transfected VHL(−) control (dark gray), **, p = 0.0001. Error bars represent mean ± S.E.