FIGURE 4.
Kinetics of CODH reduction by excess CO under anaerobic conditions. A, the kinetic trace seen at 450 nm upon mixing 12 μm CODH with 30–476 μm CO in 50 mm potassium phosphate, pH 7.2, 4 °C (transients at different CO concentrations are overlapping). Inset, the absorbance change over 1000 s. B, the kinetic traces at 520 nm at pH 7.2 and pH 9 (50 mm CHES). Curves at different CO concentrations (30–476 μm) are overlapping. C, the pH dependence of kred for CODH reduction at 4 °C.