FIGURE 6.

Kinetics of reduction of CODH with substoichiometric CO under anaerobic conditions. A, difference spectra seen in the course of the reaction of 12 μm CODH with 3.72 μm CO (at 0.02, 0.05, 0.1, 0.2, and 1 s after mixing) in 50 mm potassium phosphate, pH 7.2, 4 °C. All absorbance changes are given relative to the oxidized enzyme. B and C, kinetic transients observed at 450 nm and 520 nm, respectively, upon mixing 12 μm CODH with 3.72 or 1.86 μm CO in 50 mm potassium phosphate, pH 7.2, 4 °C.