FIGURE 4.

Point mutations in the DHR-1 domain inhibit cell elongation by Dock1. A, cells transfected with plasmids expressing wild-type or mutant Dock1, together with plasmids for ELMO1 and CrkII, were detached and plated on fibronectin-coated chambers for 2 h, and stained with anti-Dock1 antibody, rhodamine, phalloidin, and 4,6-diamidino-2-phenylindole (photographed at ×100 magnification: scale bar, 10 μm). B, quantification of cell behavior. Independent fields were photographed at lower magnification, and scored for three phenotypes: round, spread, and elongated. Data in A and B are representative of three independent experiments. C, expression levels of transfected proteins analyzed by immunoblotting cell lysates.