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. 2010 Jan 15;285(17):12482–12489. doi: 10.1074/jbc.M109.079707

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FIGURE 5. — Purification of recombinant CndG. a, lane 1, protein marker. Lane 2, whole cell extract of induced E. coli BL21, containing pGEX-CndG. Lane 3, purification of GST-tagged CndG (asterisk) by glutathione affinity chromatography. Lane 4, purified CndG (asterisk), following treatment of the GST fusion protein with PreScission Protease. b, conversion of pre-chondrochlorens 3 and 4 to the mature chondrochlorens 1 and 2, respectively, catalyzed by in vitro action of CndG, monitored by HLPC analysis over a 30-min incubation period.