FIGURE 6.

Visualization and OPIM measurement of gastrolith surface irregularities and relative transcript levels of GAP 10 in the gastrolith disc following GAP 10 silencing. A, top view of representative gastroliths, dissected from crayfish injected with ecdysone (left) or ecdysone and GAP 10 dsRNA (right). B, topographic images of representative surface samples from each group. C, real time RT-PCR relative quantification of GAP 10 transcript levels in the gastrolith disc as calculated for each experimental group injected with ecdysone (n = 5; empty bars) or ecdysone and GAP 10 dsRNA (n = 6; black bars) and mean SH as calculated for the two groups (n = 3 and n = 4, respectively). Asterisk represents statistically significant difference of p < 0.01. Two asterisk represent statistically significant difference of p < 0.001.