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. 2010 Feb 15;285(17):12684–12694. doi: 10.1074/jbc.M109.094490

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Activity of Cpz4 with synthetic liponucleoside precursor and phenol as sulfate acceptor substrates. A, conversion of pNS (%) with the different substrates (1–6 and phenol) is noted above the respective bars. Concentrations of pNS and acceptor substrates were 500 and 200 μm, respectively. B, shown is a MS² spectrum of the product 1a (m/z 878.4 [M-H]⁻, Rt. 26.2 min) formed by Cpz4 from compound 1 and pNS. The proposed fragmentation scheme for 1a is depicted. C, shown is the MS² spectrum of the product 2a (m/z 694.4 [M-H]⁻, Rt. 35.1 min) formed by Cpz4 from compound 2 and pNS. The proposed fragmentation scheme for 2a is depicted.