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. 2010 Feb 26;285(17):12559–12570. doi: 10.1074/jbc.M109.098756

FIGURE 10.

FIGURE 10.

Exogenous ADP rescues cGMP in Lyn-deficient platelets. A and B, washed platelets (3 × 108/ml) from Lyn+/+ or Lyn−/− mice were stimulated with collagen in the aggregometer for 5 min at 37 °C. They were stimulated with collagen (0.5 μg/ml) (A) or thrombin (0.025 unit/ml) (B) with or without the addition of ADP (0.5 μm). cGMP concentrations were determined using a cGMP enzyme immunoassay kit. Results are expressed as mean ± S.D. (n = 3). C, a positive feedback loop regulating platelet secretion. Platelet agonists, thrombin or collagen, bind to their receptors, which, by activating the SFK pathway, induce the activation of the PI3K/Akt pathway. PI3K/Akt induces platelet secretion via PKG. Secreted ADP activates the PI3K/Akt pathway through its Gi-coupled P2Y12 receptor, which further induces cGMP elevation.