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. 2010 Feb 23;285(17):12612–12619. doi: 10.1074/jbc.M109.082388

FIGURE 2.

FIGURE 2.

Separation of replication fork arrest and silencing functions of Fob1 in S. cerevisiae orthologs. A, two-dimensional agarose gel analysis of replication intermediates from a fob1Δ derivative of S. cerevisiae (YSB348) strain containing vector (pGAD424) alone, S. cerevisiae Fob1 (Sc Fob1), S. bayanus Fob1 (Sb Fob1), and S. paradoxus Fob1 (Sp Fob1). B, schematic diagram showing the silencing cassette (mURA3) located 50-bp downstream of the single Ter2 site present at the end of rDNA array in chromosome XII. C, silencing assay in fob1ΔYSB348 containing Fob1 orthologs. The data show that S. cerevisiae Fob1 and S. paradoxus Fob1 are proficient in silencing, but S. bayanus Fob1 is defective in silencing of the expression of the mURA3 cassette.