TABLE 3.

Rate constants

These are rate constants for ADP release in the absence and presence of mismatched DNA (mmDNA) and the rate and apparent affinity constants for release from mismatched DNA for wild type and D693N MutS in the absence and presence of magnesium. ND, not determined.

MutS	ADP exchange, koffADP		Release from mismatch
	No DNA	mmDNA	Rate	K½ATP
	s−1	s−1	s−1	mm
Wild type
Mg2+	0.0072 ± 0.0006	0.076 ± 0.029	0.24 ± 0.01	0.019 ± 0.003
EDTA	0.00068 ± 0.0	0.049 ± 0.04	0.12 ± 0.01	1.2 ± 0.2
D693N
Mg2+	0.0066 ± 0.0004	0.091 ± 0.07	0.16 ± 0.006	0.42 ± 0.05
EDTA	ND	0.033 ± 0.01	0.032 ± 0.003	0.18 ± 0.03