FIGURE 8.

Effects of kidney-specific Sirt1 overexpression on I/R-induced acute kidney injury. WT or TG mice was assaulted by renal I/R as described under “Experimental Procedures.” A, serum levels of BUN and creatinine were measured 24 h after I/R. B, pathological findings reveal extensive tubular damage with cast formation and many apoptotic bodies both in TG and WT mice. The arrows indicate the site of apoptotic bodies. Scale bar, 500 μm. C, representative images of TUNEL assay show I/R-induced renal tubular cell apoptosis in WT mice. The arrows indicate TUNEL-positive cells in high power field (×400, HPF) with condensed or fragmented nuclei. Similar levels of apoptotic cells are observed in kidneys from TG mice after I/R. Scale bar, 500 μm. **, p < 0.01 versus sham-operated WT mice; ††, p < 0.01 versus sham-operated TG mice (n = 4).