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. 2010 Feb 22;285(17):13254–13263. doi: 10.1074/jbc.M110.103051

FIGURE 6.

FIGURE 6.

The binding of MITF to the promoter of the tyrosinase gene in cells. HSP70-overexpressing B16 cells (HSP70 +) and mock transfectant control cells (HSP70 −) were incubated for 24 h with or without 100 μm IBMX. Whole cell extracts were immunoprecipitated with an antibody against MITF or HSP70. DNA fractions were prepared from the immunoprecipitated samples, and whole cell extracts and subjected to real time RT-PCR with specific primer sets for the tyrosinase promoter (upper panel) and gapdh gene (lower panel). Values are expressed relative to the input DNA (whole cell extracts) and are given as mean ± S.D. (n = 3). **, p < 0.01; *, p < 0.05.