FIGURE 3.

The Cyp2c44 epoxygenase regulates tumor growth and angiogenesis. Groups of WT and Cyp2c44^−/− (KO) mice were either left untreated of administered Wyeth (0.02%, v/v) in their drinking water for 2 days prior to receiving two subcutaneous injections with p60.5 cells. Wyeth treatment was continued for the next 2 weeks, at which point mice were sacrificed, and their tumor load quantified. A, representative images of tumors grown in untreated WT and KO mice, and in Wyeth-treated KO mice. B and C, quantification of the weight (B) and volume (C) of the tumors grown in untreated and Wyeth-treated WT and KO mice. Circles show individual tumor values, while bars show mean values. D and E, frozen sections of tumors from untreated WT and KO mice, and from Wyeth-treated KO mice were stained with anti-mouse CD31 antibodies (D), and their degrees of vascularization quantified as percent of the area occupied by CD31-positive structures per microscopic field (E). The values in E are averages ± S.D. calculated from ten tumors/group with two images analyzed per tumor.