Skip to main content
. 2010 Feb 17;285(17):13066–13078. doi: 10.1074/jbc.M109.093203

FIGURE 5.

FIGURE 5.

HET-C2 Trp emission quenching by acrylamide. Top, intrinsic Trp quenching in the presence of increasing concentrations of acrylamide (vertical arrows). Bottom, Stern-Volmer analyses of the acrylamide quenching response performed as described in the supplemental materials. The correlation coefficients are 0.999 except for LacCer/POPC vesicles (0.998). Values derived from the plots for quenching constants and Trp accessibility are summarized in Table 2.