TABLE 2.
Analysis of tryptophan fluorescence quenching
KSV and KQ are Stern-Volmer and modified Stern-Volmer quenching constants, respectively, and fa is fractional accessibility. The data represent the extent of quenching and accessibility achieved for both quenchers. The average values obtained for the quenching constants have S.D. values of 5% or less, whereas those of the fa values are less than 1%.
| Protein | Acrylamide |
KI |
Quenching |
|||||
|---|---|---|---|---|---|---|---|---|
| KSV | KQ | fa | KSV | KQ | fa | Acrylamide | KI | |
| % | % | |||||||
| HET-C2 | 19.97 | 15.4 | 1.0 | 6.9 | 7.75 | 0.97 | 90 | 76 |
| HET-C2, denatured | 15.48 | 8.8 | 1.0 | 4.48 | 8.6 | 0.72 | 87 | 70 |
| HET-C2 + POPC/GlcCer (8:2) vesicles | 10.33 | 10.7 | 0.94 | 84 | ||||
| HET-C2 + POPC/LacCer (8:2) vesicles | 12.88 | 11.5 | 0.96 | 88 | ||||
| HET-C2 + POPC vesicles | 19.45 | 12.6 | 1.0 | 88 | ||||