FIGURE 2.
Interaction of OASS and SAT as monitored by changes of fluorescence emission of PLP. Representative time courses for the reaction of OASS with SAT recorded by monitoring the fluorescence emission intensity upon excitation at 412 nm. Time courses were recorded at pH 7, 100 mm Hepes, with the concentration of OASSdimer fixed at 800 nm and the concentration of SATtrimer fixed at 80 nm. Time courses were obtained at 20 °C (black line), 12 °C (dark gray line), and 5 °C (light gray line). The dashed lines through data points represent the fit to Equation 1. Inset, fluorescence emission spectra of OASS (1.2 μm) at pH 7, 100 mm Hepes, in the absence (solid line) and presence (dashed line) of 10 μm SAT. Excitation was at 412 nm with slitex and slitem set at 5 nm. A.U., arbitrary units.