TABLE 1.

Kinetic parameters for formation of cysteine synthase

Data were obtained at pH 7. Ionic strength was 0.017 m.

	20 °C	12 °C	5 °C
k3 (s−1)	99 ± 13a	81 ± 12a	84 ± 10b
Kd (μm)	4 ± 1a	6 ± 1a	12 ± 3b
k3/Kd (m−1 s−1)c	25 ± 7a	14 ± 3a	7 ± 2b
	(13 ± 1)b	(8.7 ± 0.1)b	(4.5 ± 0.1)a

^a Parameters were obtained with OASS varied and SAT fixed. Value in parentheses were calculated from the slope of the dependence of k_obs on [OASS].

^b Parameters were obtained with SAT varied and OASS fixed. Values in parentheses were calculated from the slope of the dependence of k_obs on [SAT].

^c Values were multiplied by 10⁻⁶.