. 2010 Feb 17;285(17):12813–12822. doi: 10.1074/jbc.M109.075762

TABLE 3.

Effect of cysteine and bisulfide on kinetic parameters for formation of cysteine synthase

Data were obtained at either pH 7 (in the presence of cysteine) or pH 8 (in the presence of bisulfide).

	pH 7	+2 mm cysteine	pH 8	+1 mm bisulfide
k₃ (s⁻¹)	99 ± 13^a	57 ± 14^a	91 ± 15^a	53 ± 11^a
K_d (μm)	4 ± 1^a	5 ± 2^a	5 ± 1^a	3 ± 1^a
k₃/K_d (m⁻¹ s⁻¹)^b	25 ± 7^a	11 ± 5^a	18 ± 5^a	18 ± 7^a
	13 ± 1^c	9 ± 1^c	12.0 ± 0.2^c	13 ± 1^c

^a Parameters obtained with OASS varied and SAT fixed.

^b Values were multiplied by 10⁻⁶.

^c Parameters were obtained with SAT varied and OASS fixed. Values were calculated from the slope of the dependence of k_obs on [SAT].