Skip to main content
. 2010 Feb 24;285(17):13170–13182. doi: 10.1074/jbc.M110.108894

FIGURE 5.

FIGURE 5.

DNA alters solvent accessibility for regions of Msh2-Msh6. A, change in percent deuteration of individual peptides from Msh2-Msh6 bound to a 71-bp DNA duplex containing a central GT mispair and Msh2-Msh6 at 30 s (black) and 300 s (green). Peptides measured in both samples are shown as black and green bars spanning over the indicated sequence on the x axis. Positive and negative y axis values indicate enhancement and protection from deuterium exchange, respectively, caused by addition of the DNA substrate. Regions of substantial protection are boxed. B, change in percent deuteration of individual peptides from Msh2-Msh6 bound to a 71-bp DNA duplex containing a central GC base pair and Msh2-Msh6 at 30 and 300 s as shown in A. C, regions of solvent protection (blue) and increased solvent accessibility (red) upon DNA addition are mapped onto the ribbon diagram of Msh2-Msh6.