FIGURE 7.

Altered deuteration of the Msh2-Msh6-S1036P complex. A, change in percent deuteration of individual peptides from Msh2-Msh6-S1036P at 250 μm ATPγS at 30s (black) and 300s (green). Peptides measured in both samples are shown as black and green bars spanning over the indicated sequence on the x axis. Positive and negative y axis values indicate enhancement and protection from deuterium exchange, respectively, caused by addition of ATPγS. Regions of substantial protection are boxed. B, regions of solvent protection (blue) upon 250 μm ATPγS addition are mapped onto the ribbon diagram of Msh2-Msh6 (left) and the Msh2-Msh6 dimeric nucleotide binding domains (right).