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Purification of aminopeptidase-N from midgut brush border membrane vesicles of fourth instar M. sexta larvae
| Fraction | Total activity | Total protein | Specific activity | Recovery |
|---|---|---|---|---|
| units | mg | units/mg | % | |
| BBMV | 87.3 | 20 | 4.3 | 100 |
| BBMV/CHAPS | 49.3 | 5.4 | 9.1 | 56.4 |
| Mono Qa | 16.6 | 0.212 | 78.2 | 19.01 |
a Fig. 2 (lane 1) shows the protein profile of purified APN.
