FIGURE 1.

AIRE interacts with DAXX in yeast two-hybrid assay. Left panel, schematic representation of AIRE and DAXX constructs. A and I, full-length AIRE and DAXX. B, AIRE construct used to screen human thymus library. C–H, AIRE constructs used to map interaction domains. L, DAXX fragment that interacts with AIRE in yeast two-hybrid assay. M, DAXX construct used to map interaction domain. Right panel, quantitative two hybrid assay. α-Galactosidase units detected by the quantitative assay are shown as gray bars. The constructs used in each assay correspond to the aligned left panel. Plus and minus symbols indicate the transformant capabilities to grow on high stringency medium through ADE and HIS reporter gene activation. CC, coiled coil; NLS, nuclear localization signal; PST, Pro/Ser/Thr.