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. 2010 Feb 25;285(17):13012–13021. doi: 10.1074/jbc.M109.037747

FIGURE 3.

FIGURE 3.

HA-AIRE (aa 1–100) is ubiquitinylated in COS-1 cells. COS-1 cells were transfected with HA-AIRE (aa 1–100). After 24 h from transfection cells were treated with 2 and 5 μm MG132 (lanes 2 and 3) or left untreated (lane 1). Upper panel shows anti-HA-AIRE Western blotting (WB). Bottom panel shows the relative amounts of α-actin.