FIGURE 7.

Presence of TSA abolishes the negative influence operated by DAXX protein on the transactivation properties of AIRE. Activation of pMG3-insulin/Luc reporter in transfected HeLa. HeLa cells were cotransfected with a pMG3-insulin/Luc firefly luciferase reporter, a control Renilla luciferase reporter, equal amounts of pEF5HA-AIRE, and an increasing amounts of DAXX. Cells were left untreated for 5 h after transfection and then cultured for 24 h in presence of TSA (100 ng/ml). Cells were then lysed, and luciferase activity was measured on cell lysates. The average in ratio between firefly and Renilla luciferase activities of lysates was plotted versus AIRE and DAXX expression in same lysates as assessed by anti-HA and anti-DAXX blots.