Figure 4.

Tracking cell division in the three distinct compartments using CFSE staining. (A) Representative FACS analysis of CFSE staining at days 2 and 3. Cell generations were identified according to the control cells which were treated with mitomycin at day 0 (gray peak). (B) Distributions of cell generations of the three cell fractions at days 2, 3 and 4 (N=3). The pattern of CD34 expression in the different cell generations was studied. (C) Representative FACS analysis of CD34 and CFSE double staining at days 3 and 5. The dots in the gray region represent cells positive for CD34, the dots in the white region represent cells negative for CD34. Cell generations were identified according to the control cells which were treated with mitomycin at day 0. (D) CD34⁺ expression over eight cell generations within the three cell fractions (N = 8).