Figure 5.

(A) Enrichment of CD34⁺CD38⁻cells in the phase-dim fraction. Cells containing around 50% CD34⁺CD38⁻ cells were plated on the MSC layer. After 5 h the proportion of CD34⁺CD38⁻ cells among the phase-dim fraction was enriched up to 80% (N=4, *P<0.01). (B) Blocking experiments for P-selectin, integrin β1 and CXCR4. By blocking integrin β1 or CXCR4 or both, percentages of phase-bright or phase-dim cells were down-regulated (N = 4, *P<0.01 (relative to control); #P<0.05 (relative to individual blocking for integrin β1 or CXCR4).