Figure 2.

Group I mGluR-mediated APP processing in the synapse. A, Cortical synaptoneurosomes were stimulated with 100 μm DHPG (group I mGluR agonist), and synaptoneurosome-associated CTF levels were measured by Western blot. DHPG stimulation caused rapid accumulation of C83 and C99, indicating activation of α- and β-secretases. In the representative Western blot image, “DCG-IV”-labeled bands are for Figure 3 A. The values were normalized to the baseline (t = 0). n = 7 for each. B, Group I mGluR stimulation triggered release of Aβ₄₀ but not Aβ₄₂ from synaptoneurosomes. The values were normalized to the baseline (t = 0). n = 4 for Aβ₄₀; n = 8 for Aβ₄₂. Data are presented as means ± SEM. *p < 0.05; **p < 0.01; ^# p < 0.001.