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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: J Comp Neurol. 2010 Jan 1;518(1):6–24. doi: 10.1002/cne.22221

Figure 1.

Figure 1

Immunohistochemical labeling of GFP-positive neurons in the left nodose ganglion. Many GFP-positive neuronal somas and fibers were observed in DAB-labeled (brightfield) (B) and fluorescently-labeled sections (epifluorescence and Apotome) (C). Omission of the primary antibody resulted only in residual background staining (A). Higher magnification of DAPI-counterstained sections reveals neurons (large cellular nuclei marked by asterisks), a number of which show GFP immunoreactivity (D). Note that GFP-positive neurons vary in size. Coexpression of MC4R mRNA and GFP in the left nodose ganglion (E,F). Neurons with clusters of silver grains are visible throughout the ganglion (darkfield) (E). GFP-positive neurons (stained in brown) are overlaid by black silver grains which indicate MC4R mRNA expression (brightfield) (F). Black arrows show examples of colocalization. The black arrowhead shows one example of a GFP-positive profile devoid of hybridization signal. The doubled-headed arrows indicate the central (c) versus the peripheral (p) tip of the ganglion.