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. Author manuscript; available in PMC: 2011 Mar 2.
Published in final edited form as: Biochemistry. 2010 Mar 2;49(8):1649–1657. doi: 10.1021/bi902084y

Figure 2.

Figure 2

Binding of Stxs to Pk analogues in ELISA and SPR platforms. A, Binding of Stxs to Pk in ELISA and SPR platforms. B, Binding of Stxs to NHAc-Pk in ELISA and SPR platforms. Error bars indicate mean ± SEM of three independent experiments. ELISA binding is expressed as % of the value obtained for highest toxin concentration tested for each plate. SPR binding is expressed in response units (RU) at 200 sec after injection. Molar concentrations for Stx B-subunits are based upon MW of pentamer. The chemical structures of the biantennary Pk (A) and NHAc-Pk (B) glycoconjugates immobilized on the ELISA and SPR surfaces are indicated.