Figure 8.

TCR:CD3 ubiquitylation modulates T-cell development. (A–C) Bone marrow from female or male CD3ζɛ^−/−Rag1^−/− mice was co-transduced with the MataHari TCRαβ and CD3^WT, CD3^KR, or CD3^KR-monoUb. Transduced bone marrow was used to reconstitute irradiated, sex-matched Rag1^−/− mice. Mice were bled and killed 6–8 weeks after transfer. Peripheral blood was stained with antibodies to CD8 and Vβ8.3 (A). Bar charts show the number of thymocytes (B) and CD5 expression on DP thymocytes (C) in each group. Data are gated on GFP⁺ cells and are represent the mean±s.e.m. of 5–10 mice from two experiments per group. A full-colour version of this figure is available at The EMBO Journal Online.